《中国神经再生研究（英文版）》杂志于2012年11月32期出版的一项关于“Tacrolimus reduces scar formation and promotes sciatic nerve regeneration”的研究发现，①他克莫司可减少坐骨神经损伤大鼠神经吻合口胶原纤维含量和瘢痕面积。②他克莫司可增加坐骨神经损伤大鼠神经吻合口有髓神经纤维密度、平均轴突直径和髓鞘厚度。③他克莫司可促进坐骨神经损伤大鼠神经功能的恢复。④大鼠坐骨神经有髓神经纤维密度及坐骨神经功能指数与瘢痕面积呈负相关。作者认为，他克莫司通过减少瘢痕形成促进周围神经再生，加快周围神经功能恢复。
Tacrolimus reduces scar formation and promotes sciatic nerve regeneration
Jun Que1, Quan Cao1, Tao Sui2, Shihao Du2, Ailiang Zhang2, Dechao Kong2, Xiaojian Cao2
A sciatic nerve transection and repair model was established in Sprague-Dawley rats by transecting the tendon of obturator internus muscle in the greater sciatic foramen and suturing with nylon sutures. The models were treated with tacrolimus gavage (4 mg/kg per day) for 0, 2, 4 and 6 weeks. Specimens were harvested at 6 weeks of intragastric administration. Masson staining revealed that the collagen fiber content and scar area in the nerve anastomosis of the sciatic nerve injury rats were significantly reduced after tacrolimus administration. Hematoxylin-eosin staining showed that tacrolimus significantly increased myelinated nerve fiber density, average axon diameter and myelin sheath thickness. Intragastric administration of tacrolimus also led to a significant increase in the recovery rate of gastrocnemius muscle wet weight and the sciatic functional index after sciatic nerve injury. The above indices were most significantly improved at 6 weeks after of tacrolimus gavage. The myelinated nerve fiber density in the nerve anastomosis and the sciatic nerve functions had a significant negative correlation with the scar area, as detected by Spearman’s rank correlation analysis. These findings indicate that tacrolimus can promote peripheral nerve regeneration and accelerate the recovery of neurological function through the reduction of scar formation.
